Abstract
Plantaricin LPL-1, produced by Lactobacillus plantarum LPL-1, is a novel class IIa bacteriocin with bactericidal activity against foodborne bacteria. The aim of this present study was to understand the antibacterial activity and mechanism of plantaricin LPL-1 against Listeria monocytogenes 54002, one of the Gram-positive bacteria sensitive to this bacteriocin and typical foodborne pathogenic bacteria in the food industry. The minimal inhibitory concentration (MIC) was confirmed as 16 μg/mL, and the time-kill kinetics showed that plantaricin LPL-1 significantly decrease the viable cell numbers of L. monocytogenes 54002 in a time-dependent and dose-dependent manner. The antibacterial mechanism was investigated using confocal laser-scanning microscopy, scanning and transmission electron microscopy, spectrofluorimeter, spectrophotometer and Real-Time PCR system, which demonstrated that plantaricin LPL-1 facilitated pores formation, damaged cell membrane integrity, increased membrane permeability, triggered intracellular material leakage, induced collapse of proton motive force, and inhibited expression levels of genes related to virulence factors, biofilm formation factors, and RNA polymerase sigma factor. Hence, the bactericidal mechanism of plantaricin LPL-1 against L. monocytogenes was clarified at cellular and molecular levels.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.