ANTIBACTERIAL EFFICACY OF STREPTOMYCES FRADIAE BUCBTPVD9 FROM MARINE AGAINST CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIA

Abstract

The emergence of drug resistance is one of the major threats which is now becoming an alarming situation around the world. The UTI causing organisms are also becoming resistant towards the available treatment protocols. Hence the discovery of new, efcient antibacterial metabolites is in need. Actinobacteria has its proven records in the eld of pharmaceutical industry, the secondary metabolites are potent towards the resistant strains. The aim of this study is to identify the antibacterial metabolite producing actinobacterial strains from estuary soil. Study focused on collecting soil samples from estuary in Kerala and Tamil Nadu. From the collected soil samples around nine actinobacterial strains were isolated. Among the nine different strains one strain BUCBT-PVD9 was found to be effective against all the UTI MDR strains which was taken for further study. On characterizing the potent strain it was evident that the strain belongs to Streptomyces fradiae, based on biochemical, spore arrangements and molecular characterization. Further the one factor optimization was performed to check the inuencing factor for enhanced antibacterial metabolite production. Factors like pH, temperature, Nitrogen & Carbon sources along with incubation period on SSY media which showed increased production of metabolite was optimized to increase the yield of the active metabolite. MIC of the ethyl acetate fraction was determined and found to be 31.25 µg/ml against CRKP and 250 µg/ml against ATCC 1705. Further studies are required to characterize the active metabolite which is responsible for the antibacterial activity.