Abstract
Medicinal and pesticidal properties of neem (Aadirachta indica) have been described for more than 4,000 years. In the present study, antibacterial properties of Aadirachta indica seed oil have been investigated against Escherichia coli, Bacillus subtilus, Rizobium meliloti and Pseudomonas aeruginosa by disc diffusion method. At 100% concentration, acetone soluble oil possessed the highest antibacterial activity causing 18 mm zone of inhibition against E. coli and 17 mm against P. aeruginosa as compared to ciprofloxacin used as a positive control. The 1inhibition zone showed a significant difference across all the bacteria (p<0.01) and concentrations (p<0.001). This study focuses the use of neem oil as an alternative to ciprofloxacin.
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