Antibacterial effects of gallic acid loaded graphene oxide (GAGO) against methicillin-resistant Staphylococcus aureus (MRSA)

Abstract

Event Abstract Back to Event Antibacterial effects of gallic acid loaded graphene oxide (GAGO) against methicillin-resistant Staphylococcus aureus (MRSA) Nurhuda Elias1, Seri Narti Edayu Sarchio2, Faizah Md Yasin3, Shafinaz Abdul Gani1 and Suhaili Shamsi1* 1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia 2 Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Malaysia 3 Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Malaysia Background Staphylococcus aureus strains known as “superbugs” are exceptionally rigid and can quickly develop protective mechanisms against synthetic antibiotics. One increasingly common superbug is methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to most available antibiotics. With the ongoing dearth in antibiotics development, the design and identification of alternative classes of antibacterial agents with new modes of action that can effectively overcome multidrug resistance (MDR) is more pressing than ever. The present study aims to investigate the potential of graphene oxide (GO), a biocompatible and cost-effective graphene derivative, with promising antibacterial properties, and gallic acid, a phenolic compound with myriad health benefits, including antibacterial activity, combined together by means of nanoformulation (GAGO) to reverse the MDR in MRSA, hence enhancing antibacterial efficacy. The present study evaluated the interaction of GAGO with MRSA, and its capacity to exert antibacterial activity against MRSA. Methods GAGO was synthesized by physical mixing of GO and GA at 1: 5 ratio (GO: GA) and was characterized for morphology, chemical characteristics and GA content. The interaction of GAGO with MRSA was investigated by evaluating its antibacterial activity through disc diffusion and minimum inhibitory concentration (MIC) assays. Results GA was successfully loaded onto GO (GAGO) with a loading efficiency of 29.11% that resulted in a GA loading of 886 mg/g of GAGO nanocomposite. The GAGO obtained was in the form of black thin film and FESEM image showed the thickness of the GAGO structure, as compared to only GO which indicates the loading of GA. The antibacterial activity of GA against MRSA increased significantly at lower concentration when loaded onto GAGO nanoformulation (MIC: 150 µg/mL to 100 µg/mL) due to the presence of GO. Conclusion Fundamentally, the results demonstrated the potential of GAGO as a promising antibacterial agent against MRSA. Acknowledgements This research is supported by grant from the Universiti Putra Malaysia (GP-IPS/2018/9602000). The authors acknowledge the facilities, scientific and technical assistance of Institute of Advanced Technology (ITMA) at Universiti Putra Malaysia. Keywords: graphene oxide, Gallic Acid, Antibacterial, multi-drug resistance, MRSA Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Infectious diseases Citation: Elias N, Sarchio S, Md Yasin F, Abdul Gani S and Shamsi S (2019). Antibacterial effects of gallic acid loaded graphene oxide (GAGO) against methicillin-resistant Staphylococcus aureus (MRSA). Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00108 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Suhaili Shamsi, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia, sh_suhaili@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nurhuda Elias Seri Narti Edayu Sarchio Faizah Md Yasin Shafinaz Abdul Gani Suhaili Shamsi Google Nurhuda Elias Seri Narti Edayu Sarchio Faizah Md Yasin Shafinaz Abdul Gani Suhaili Shamsi Google Scholar Nurhuda Elias Seri Narti Edayu Sarchio Faizah Md Yasin Shafinaz Abdul Gani Suhaili Shamsi PubMed Nurhuda Elias Seri Narti Edayu Sarchio Faizah Md Yasin Shafinaz Abdul Gani Suhaili Shamsi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.