Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study.

Silvana Enciso,Frank Mayta-Tovalino,Luzmila Vilchez,Daniel Alvitez-Temoche,Cesar Mauricio-Vilchez,Julia Medina,Franco Mauricio

doi:10.1155/2020/8856382

Abstract

ObjectiveTo determine the in vitro antibacterial effect of four concentrations of the hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) against Streptococcus mutans ATCC 25175TM. MethodsThis was a prospective, experimental, comparative study. Fermented tocosh was subjected to hydric stress to obtain a hydroalcoholic extract at four different concentrations: 100%, 50%, 75%, and 25%. S. mutans strains were cultured in brain heart infusion agar using the swab technique. The antibacterial effectiveness of HET was evaluated following the Kirby–Bauer disk diffusion method and compared with 0.12% chlorhexidine (positive control group).ResultsThe highest mean inhibitory effect was achieved with HET at 100% (33.1 ± 2.2 mm, showing a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)). Inferential analysis found statistically significant differences among all the experimental groups (p=0.001). The post hoc analysis also showed significant differences among all the experimental groups evaluated; however, there were no significant differences between HET 50% and chlorhexidine 0.12% (p > 0.05).ConclusionsIt was found that the highest antibacterial effectiveness was obtained by HET 100%, being even higher than the 0.12% chlorhexidine positive control, and was statistically significant. Post hoc analysis showed that almost all the concentrations showed optimal efficacy against S. mutans.

Highlights

Evaluation of In Vitro Antimicrobial Activity. e highest mean inhibitory effect was obtained with hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) at 100% (33.1 ± 2.2 mm), with a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)
No differences were observed between HET 50% and chlorhexidine 0.12% (p > 0.05) (Table 1)
It is original, and there is a lack of studies on the antibacterial activity of Solanum tuberosum “tocosh” in S. mutans, and the results of this study provide a scientific basis for the use of tocosh as an alternative therapy in the control of oral diseases. is study is important in the search for alternative products with proven antimicrobial action, which can be obtained from the flora of Peru

Summary

Methods

Is experimental, prospective, comparative study was carried out in the Microbiology Laboratories of the Faculty of Natural Sciences of Universidad Nacional Federico Villarreal and the Laboratory of Plant Anatomy and Pharmacognosy of Universidad Nacional Mayor de San Marcos. E sample provided was taken to the Laboratory of Plant Anatomy and Pharmacognosy and stored in a sterile glass container weighing 2 kg. En, the 60° hydroalcoholic solution was prepared from 96° ethyl alcohol and bidistilled water. E sample was placed into two 20 L glass containers, maintaining a ratio of 1 : 10 of the sample/volume of hydroalcoholic solution (1 kg of sample/10 L of solution), for a period of 10 days. E entire procedure was carried out in the Plant Anatomy and Pharmacognosy Laboratory of the Faculty of Biological Sciences of the National University of San Marcos with voucher no.

Results

Discussion

Conclusion