The impact of hydroalcoholic extract of Anacyclus pyrethrum plant on epileptic seizure induced by pentylenetetrazole in male rat

Abstract

BackgroundThe aim was to evaluate the protective effect of hydroalcoholic extract of Anacyclus pyrethrum root (APE) against pentylenetetrazole (PTZ) drug which is used for inducing epileptic seizures in animal model.Results50 male rats were divided: control (without any intervention), positive control 1st (received PTZ 60 mg/kg, IP), first experimental group (PTZ + Extract 500 mg/kg, gavages, 30 min before PTZ), positive control 2nd (PTZ + Phaclofen, 200 µg/µl, ICV), and second experimental group (PTZ + extract 500 mg/kg, gavage, 30 min before PTZ + Phaclofen 200 µg/µl, ICV). Several parameters were assessed during 20 min and followed up for 1.5 h. Then, the data were analyzed. APE with a dose of 500 mg/kg increased the latency time of seizures in the first experimental group, compared to the positive control 1st, also, comparison of different groups in terms of Seizure Score at the 1st time (severity of first attack) had no significant difference (P-value = 0.51, P-value = 0.34). The mean of seizure attacks (event number) was significant between the first and second positive control groups (P-value = 0.01) and also between the second positive control and the first experimental group (P-value = 0.011). Significant changes were observed in the mean score of the first and second positive control groups (P-value = 0.001) and the first experimental and second positive control groups (P-value = 0.003). In addition, the second experimental group had significant changes compared to the first positive control group (P-value = 0.014), However, no significant changes were observed between the positive control and experimental groups in terms of the severity of seizures.ConclusionResults have shown both blocked GABAergic receptors A and B involved in epileptic seizures. In addition, APE root increased delay time of epileptic seizures, as well as reduces epileptic seizure in dose response state.