Abstract
Theamethanolicoextractsaofffiveeaerial parts ofaMoringa oleifera Lamwwereascreened for their antibacterial, antibiofilm and hemolytic activity.”Theaphytochemical screening of the methanolic extracts of aerial parts was also evaluated. The antibacterial, antibiofilmiactivityiof the extractssof various aerial parts of M. oleifera was evaluated by disc diffusion and micro titer plate assay method against two selected bacterial species. Hemolyticaactivity of the methanoliciextracts was screened against normal human erythrocytes. Comparatively the methanolic extracts of all aerial parts were more effective against Gram positive bacteria Bacillus subtilis and Gram negative bacteria Escherichia coli (E. coli) .The present study suggests that all aerial parts of this plant can be used as an antibacterial, anti-biofilm agent in treating ailments caused by the tested organisms (Bacillus subtilis and Escherichia coli). Methanolic extracts of aerial parts possessed minimum hemolytic activity. Significant antimicrobial, antibiofilm activity of the extract was found in this study.Asian J. Med. Biol. Res. March 2018, 4(1): 77-85
Highlights
The use of natural substances in the treatment of different diseases, including those of infectious disease is currently a challenge in medicine and offered an alternatives, especially in those ailments for which there is no adequate remedy (Domingo and López-Brea, 2003)
Anti-biofilmaactivity of the aerial parts The effectaof the methanoliccextracts of the aerial parts of Moringa oleiferason biofilm formationawas examinedafollowing the method of (Yarwood et al, 2004) micro titer plate assay with a little modification.”The appropriate concentration of plant extracts was added to the wells of 96 microtiter plates having bacterial cultures inoculated in LB (Luria-Bertani) broth.”Negative and positive controls were included in the experiment.Plates were incubated for 24 h at 37°C
The result showed that the methanolic extracts of various aerial parts of M. oleifera showed variable antibacterial activity against selected bacterial pathogens equivalent to that of standard against the entire tested organism
Summary
The use of natural substances in the treatment of different diseases, including those of infectious disease is currently a challenge in medicine and offered an alternatives, especially in those ailments for which there is no adequate remedy (Domingo and López-Brea, 2003). They are rich in a wide variety of secondary metabolites such as tannins, alkaloids flavonoids, vitamins (A, C, E and K), carotenoids, terpenoids, polyphenols, saponins, pigments, enzymes and minerals that have antimicrobial and antioxidant activity (Khan et al, 2009). Moringa oleifera is known to possess hypoglycemic, hypotensive, antimicrobial, hepatoprotective, immunomodulatory, antioxidant and antitumor activities (Mahajan et al, 2010; Sudha et al, 2010). It is one of the best known, widely distributed and grown species of a monogeneric family. Moringa oleifera has been reported to possess antimicrobial properties and this explains the reason for its extensive use in the treatment of human ailments (Lockettet et al, 2000). Theiobjective of the currentistudy was,itherefore, to determine the antibacterial, antibiofilmahemolyticaactivitiesaand phytochemical study of aerial parts of Moringa oleifera
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