Antibacterial, Antibiofilm, Antiquorum Sensing, Antimotility, and Antioxidant Activities of Green Fabricated Ag, Cu, TiO2, ZnO, and Fe3O4 NPs via Protoparmeliopsis muralis Lichen Aqueous Extract against Multi-Drug-Resistant Bacteria.

Abstract

Consideration of lichen organisms as the ecofriendly source of metal nanoparticles (MNPs) and metal oxide NPs (MONPs) synthesis is seldom. In this study, Ag and Cu MNPs as well as TiO2, ZnO, and Fe3O4 MONPs were green synthesized by Protoparmeliopsis muralis lichen aqueous extract. First, physicochemical characterization by UV-vis spectroscopy, XRD, FT-IR, FESEM, and TEM techniques demonstrated the presence possibility of secondary metabolites around formed MNPs/MONPs with different diameters and shapes (spherical, triangular, polyhedral, and cubic). The antibacterial, antibiofilm, antiquorum sensing, and antioxidant abilities of these MNPs/MONPs against multi drug resistant (MDR) bacterium (Staphylococcus aureus ATCC 43300) and reference bacteria (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) were then evaluated by in vitro tests. Results of disc diffusion and MIC/MBC assays of Ag NPs as an effective antibacterial agent illustrated a higher sensitivity of the P. aeruginosa pathogen than E. coli and S. aureus. In next steps, a significant reduction was observed in the biofilm formation of each bacterium and pyocyanin synthesis by P. aeruginosa under Ag NPs. This investigation presents novel clean production of five MNPs/MONPs with prominent advantages of being ecofriendly and cost-effective and having antipathogen properties.