Kinetic profile of silver and zinc oxide nanoparticles by intraperitoneal injection in mice , a comparative study

Sarab Mohammed M Razooki,Adel M Rabee

doi:10.21533/pen.v7i3.799

Abstract

To investigate the kinetic profile (Absorption , distribution , and excretion ) of intraperitoneal injected Ag NPs (24.52 nm ) and ZnO NPs (25.16 nm) in Albino mice , a single dose of the two kinds of NPs (100 mg/kg) were used for comparison .Kinetic profile in blood clarified shorter time ( T max ) were spend by ZnO NPs to be absorbed compared with longer one recorded by Ag NPs as their smaller volume of distribution (Vd) to confirm their higher concentration (C max ) with 95 µg/ml compared with Ag NPs which stands on 57.5 µg/ml , also Ag NPs needs longer time of elimination to their half values (t1/2 Elem.) in blood with lower clearance rate (CL) compared with ZnO NPs . This study discussed the distribution profile in different organs over time and pointed a considerable Ag and Zn concentrations specially in liver , spleen , intestine and kidney, also an important levels recorded in lung ,heart , testes and brain. Ag and ZnO NPs excretion manner noted a significant percentage of excretion via feces with 33 % and 83%for Ag NPs and ZnO NPs respectively compared with small percentage recorded with urine .

Highlights

Nano industry has been described as the industrial revolution as turning to be one of the fastest growing industries in the history of human beings [1] with expanding nanotechnology scope in continuous manner as using of nano materials in many implementations in our daily life [2]
Characterization of Ag and ZnO NPs According to the granularity distribution chart, the average diameter for Ag and ZnO NPs samples were 24.52 nm, 25.16 nm respectively with spherical shape determined for the two types of NPs using atomic force microscope (AFM) (Figure 1)
Lower value of maximum concentration detected in blood referred to silver compared with zinc which may caused by molecular weight variation [24], the probable aggregations of Ag NPs as the postponement in the peritoneal cavity leading to increase the surface area [23] and reduce the amount of silver that can pass through peritoneal membrane, in addition to and amount of generated ions [21], [25] and some considerable effects like lymphatic drainage, peritoneal permeability, and particle charge [26], [27]

Summary

Introduction

Nano industry has been described as the industrial revolution as turning to be one of the fastest growing industries in the history of human beings [1] with expanding nanotechnology scope in continuous manner as using of nano materials in many implementations in our daily life [2]. Nanoparticles are defined as one or more dimension small objects in range of 1–100 nm. Due to their small size and large- scale surface area compared with micro scale , different degrees of biological toxic effects were demonstrated by large numbers of studies [3]-[6]. Among metal containing nanoparticles , zinc oxide (ZnO NPs) was the third highest production over the world with 550 tons, widely used in cosmetic products as UV light scatter specially in sun screens , and dermal ointments, and toothpastes [8]. There are real needs to protect our public and environmental health and safety, specially where the standards or guidelines that can directly rule

Methods

Results

Conclusion