Abstract
The provision of nanoparticles using biogenic material as a part of green chemistry is an attractive nanotechnology. The current research aimed to test the antimicrobial and cytotoxic efficacy of silver nanoparticles synthesized by extracts of Phoenix dactylifera, Ferula asafetida, and Acacia nilotica as reductant and stabilizing agents in silver nanoparticle formation. Synthesized nanoparticles were evaluated for their antimicrobial activity against Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa and Escherichia coli (Gram-negative) using an agar well diffusion assay. Furthermore, cytotoxic ability was investigated against LoVo cells. The potential phyto-constituents of plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques. Field emission scanning electron microscopy (FE-SEM), transmission electron microscope (TEM), and zeta potential analyzed the size and morphology of the biogenic nanoparticles. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average size that ranged between 67.8 ± 0.3 and 155.7 ± 1.5 nm in diameter. Biogenic AgNPs showed significant antibacterial ability (10 to 32 mm diameter) and anticancer ability against a LoVo cell with IC50 ranged between 35.15–56.73 μg/mL. The innovation of the present study is that the green synthesis of NPs, which is simple and cost effective, provides stable nano-materials and can be an alternative for the large-scale synthesis of silver nanoparticles.
Highlights
Worldwide problems associated with environmental concerns have led to a focus on the technology of green chemistry as an environmentally friendly process in chemistry to overcome different health problems [1]
Different microbes were studied as the bio-mediator in silver nanoparticle formation such as E. coli [17], a cell-free supernatant derived from Bacillus sp. culture [18], Aspergillus flavus, Lactic acid bacteria, Bacillus licheniformis, and Bacillus cereus, [19,20,21,22], but plant extracts showed a higher ability for the conversion of Ag ions to AgNPs compared to conversion mediated by microorganisms [23]
Plant extracts and their high potentiality in AgNPs biosynthesis, beside their antibacterial and cytotoxicity, were the aims of the current investigation since the plant is a rich source of phytochemicals
Summary
Worldwide problems associated with environmental concerns have led to a focus on the technology of green chemistry as an environmentally friendly process in chemistry to overcome different health problems [1]. Culture [18], Aspergillus flavus, Lactic acid bacteria, Bacillus licheniformis, and Bacillus cereus, [19,20,21,22], but plant extracts showed a higher ability for the conversion of Ag ions to AgNPs compared to conversion mediated by microorganisms [23] This might be due to the fact that plants are a rich source of biologically active compounds such as flavones, ketones, aldehydes, amides, carboxylic acids, proteins, DNA, and enzymes. A recent study suggested the mechanism of AgNPs on bacterial cell death is its influence on membrane morphology that leads to improper permeability and material transport [28] Plant extracts and their high potentiality in AgNPs biosynthesis, beside their antibacterial and cytotoxicity, were the aims of the current investigation since the plant is a rich source of phytochemicals. The cytotoxic effect was determined against LoVo cell lines by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assay
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
