Abstract
The study was conducted to investigate the antibacterial and antioxidant effects of Padina australis Hauck crude extract. The essential components of the brown algae was obtained by ethanolic extraction and analyzed by Thin Layer Chromatography (TLC) Silica gel plates. The antibacterial activities of the algal extract at different concentrations were determined using the Kirby Bauer Method and were expressed as zone of inhibition. The algal extract showed to have antibacterial effects on Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Neisseria gonorrhoeae. The antioxidant activity of the algal extract was determined using the methods of inhibition on DPPH Free Radical. The antioxidant effects of the extract at different concentrations were compared with standard antioxidant, such as Ascorbic Acid. The algal extract was found to be active in the decolorization of Methanolic-DPPH solution and scavenging the free radical. The antibacterial and antioxidant activities could be attributed to the presence of different secondary metabolites such as phenolic compounds, and carotenoids and the mechanism of action might be due to their individual or collective participation. The experimental findings envisaged P. australis extract as a good candidate in developing new antibacterial and antioxidant agents. Keywords – Algae, Padina australis, DPPH Free Radical, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Neisseria gonorrhoeae, Philippines
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