Antibacterial Activity Tests of Staphylococcus aureus and Phytochemical Screening in Family Asteraceae, Clusiaceae, Phyllanthaceae

Abstract

Infection is a type of disease that affects many residents of developing countries, including Indonesia. One pathogenic bacteria that is quite dangerous and causes skin infections both sporadically and endemically is S aureus. Several studies intensively report that some of the Asteraceae, Clusiaceae and Phyllanthaceae family plants contain alkaloids, flavonoids, saponins and tannins that have potential as antibacterial S aureus. Therefore, this study aims to identify effective plants to deal with infections caused by S aureus bacteria. To this end, the ingredients used are the leaves of G. procumbens, E. scaber, G. mangostana, G atroviridis, G. xanthochymus, leaves and bark of A. neurocarpum. Plant material extraction was done by maceration using 90% ethanol solvent. Next, an antibacterial test was conducted, which was begun by testing the Minimum Inhibitory Concentration followed by testing the Obstacle Area Width. The Minimum Inhibitory Concentration test was carried out using the agar dilution method, and the Obstacle Area Width test was carried out with paper diffusion discs. The results showed that all the plants have potential as antibacterials, and the mangosteen leaf extract has the largest Obstacle Area Width of 5.1 mm with a concentration of extract of 30% and a fairly strong inhibitory ability.