Antibacterial Activity of Volatile Organic Compounds Produced by the Octocoral-Associated Bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327.

Anette Garrido,Marcelino Gutiérrez,Rita Bethancourt,Armando A Durant-Archibold,Librada A Atencio,Ariadna Bethancourt,Héctor Guzmán

doi:10.3390/antibiotics9120923

Abstract

The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327. The volatilome bioactivity of both bacteria species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, and Pseudomonas aeruginosa. In this regard, the in vitro tests showed that Bacillus sp. BO53 VOCs inhibited the growth of P. aeruginosa and reduced the growth of S. aureus and A. baumanni. Furthermore, Pseudoalteromonas sp. GA327 strongly inhibited the growth of A. baumanni, and P. aeruginosa. VOCs were analyzed by headspace solid-phase microextraction (HS-SPME) joined to gas chromatography-mass spectrometry (GC-MS) methodology. Nineteen VOCs were identified, where 5-acetyl-2-methylpyridine, 2-butanone, and 2-nonanone were the major compounds identified on Bacillus sp. BO53 VOCs; while 1-pentanol, 2-butanone, and butyl formate were the primary volatile compounds detected in Pseudoalteromonas sp. GA327. We proposed that the observed bioactivity is mainly due to the efficient inhibitory biochemical mechanisms of alcohols and ketones upon antibiotic-resistant bacteria. This is the first report which describes the antibacterial activity of VOCs emitted by octocoral-associated bacteria.

Highlights

The fight against bacterial infections has been one the focus of attention of humanity. in the 20th century an important number of discovered antibacterial compounds have improved the quality of life of the people, it has been observed an increased antibiotic resistance prevalence among bacteria which represents the greatest challenge to human health
In the 20th century an important number of discovered antibacterial compounds have improved the quality of life of the people, it has been observed an increased antibiotic resistance prevalence among bacteria which represents the greatest challenge to human health
Antibacterial Activity of volatile organic compounds (VOCs) Produced by Bacillus sp

Summary

Introduction

The fight against bacterial infections has been one the focus of attention of humanity. In the 20th century an important number of discovered antibacterial compounds have improved the quality of life of the people, it has been observed an increased antibiotic resistance prevalence among bacteria which represents the greatest challenge to human health. The main molecular mechanisms of bacteria resistance to antibiotics are due to mutations in bacterial genes; and due to the elevated number of multidrug resistance pumps (MDR pumps), which extrudes. P. aeruginosa is an opportunistic pathogen that is a leading cause of morbidity and mortality in cystic fibrosis patients and immunocompromised individuals. It is one of the main risk factors for nosocomial infections and ventilator-associated pneumonia [6]

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