Antibacterial activity of Syzygium aromaticum against Klebsiella pneumoniae and Staphylococcus aureus isolated from urinary tract of dogs

Abstract

This study was conducted to investigate the antibacterial efficacy of Syzygium aromaticum extracts, (clove) is one of the traditional plants that have been used to prevent urinary tract infection. Our study showed the antibacterial effectiveness of the extract of Syzygies aromaticum and reduced bacterial resistance resulting from the misuse of the antibiotic. Our extract has been used to prevent UTI because the pharmacological activities are attributed to plant constituents such as flavonoids, alkaloids, glycosides, saponins, terpenes, and other active ingredients. Cloves were extracted by hydroalcoholic extract (ethanol), which yield a reddish-brown plant extract powder with 20%. It was observed that the hydroalcoholic extract (ethanol)of cloves against Staphylococcus aureus appeared at a concentration of 6.25 mg/ml, and increased until reached concentrations of 200; 400 and 800 mg/ml resulting in a zone of inhibition (16.3, 17.5, 20.7mm), respectively, while inhibition was recorded against Klebsiella pneumoniae at a concentration of 50 mg/ml. At a concentration of 400 and 800 mg/ml, an increase in inhibition was observed for the zone (18.1, 22.3mm). It was inferred that the hydroalcoholic extract (ethanol)of cloves has a higher antibacterial effect against Staphylococcus aureus because the zones of inhibition began with lower concentrations compared to Klebsiella pneumonia because of their virulence factors in addition to bacterial resistance