Antibacterial Activity of Silver Nanoparticles Poguntano Herb Extract (Picria fel-terrae Lour) against Staphylococcus epidermidis

Abstract

Green synthesis methods to synthesize nanoparticles using natural products can be used by utilizing plants or microorganisms. Poguntano (Picria fel-terrae Lour.) or leaf kukurang is a plant from the family Scrophulariaceae. This study aimed to determine the antibacterial activity of silver nanoparticles of Poguntano aqueous extract. The antibacterial activity of silver nanoparticle water extract bacteria Staphylococcus epidermidis with extract concentrations of 20 mg/mL (12.43 ± 0.12), 10 mg/mL (11.50 ± 0.10) and 5 mg/mL (10.43 ± 0.12) can inhibit bacterial growth, Staphylococcus epidermidis at a concentration of 10 mg/mL, absorbance values at wavelengths of 260 nm and 280 nm were higher than the absorbance values at a concentration of 5 mg/mL. Staphylococcus epidermidis biofilm inhibition test shows that the results of the absorbance or OD measurement in the biofilm inhibition test using the silver nanoparticle of Poguntano extract, the OD value at a concentration of 10 mg/mL (0.367± 0.57) showed a biofilm inhibition result of 45.24 % greater than the concentration of 5 mg/mL which is 0.510 ± 0.54 with a biofilm inhibition result of 23.77%, this indicates that the greater the attention of the extract, the smaller the biofilm formation of Staphylococcus epidermidis bacteria.