Antibacterial activity of silver nanoparticles Clinacanthus nutans (Agnp-CN) against Streptococcus mutans

Abstract

Event Abstract Back to Event Antibacterial activity of silver nanoparticles Clinacanthus nutans (Agnp-CN) against Streptococcus mutans Siti Aisyah Abd Ghafar1*, Nor Baitie Adura Muhd Fudzi1, Wan Nur Farhanah Wan Sulaiman1, Rohazila Mohamad Hanafiah1 and Vuanghao Lim2 1 Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Malaysia 2 Cluster of Integrative Medicine, Advanced Medical and Dental Institute, University of Science, Malaysia, Malaysia Background Clinacanthus nutans was found to possess anti-venom, anti-inflammatory, analgesic, anti- diabetic, anti-rheumatism, antiviral and antioxidant properties. Silver nanoparticles are nanoparticles between 1nm to 100nm in size and play significant role in medicinal fields. Silver nanoparticles exhibit distinctive properties, such as good conductivity, chemical stability, catalytic and antibacterial activity. Streptococcus mutans is commonly found in human oral cavity and the main contributor to tooth decay. There is no study on antibacterial effect of silver nanoparticles Clinacanthus nutans (AgNp-CN) against Streptococcus mutans reported to date. Therefore, objective of this study is to investigate antibacterial properties of silver nanoparticles Clinacanthus nutans against Streptococcus mutans. Methods Streptococcus mutans was subcultured in brain heart infusion (BHI) broth and agar. AgNp-CN with different concentrations was tested against Streptococcus mutans via disc diffusion assay, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Amoxicillin was used as positive control while DMSO and blank silver nanoparticles were used as negative control. Results Disc diffusion assay shows AgNp-CN inhibit Streptococcus mutans growth. AgNp-CN shows the greatest inhibition properties (15.3±1.53mm) in comparison to Clinacanthus nutans leaves extract (6.0±0.00mm) and blank silver nanoparticles (6.0±0.00mm). MIC value for AgNp-CN is 2.5mg/mL while amoxicillin is 25mg/mL. Whereas MBC values for AgNp-CN is 2.5mg/mL. Results are concentration dependent, with higher concentration shows better inhibition property. Conclusion It can be concluded AgNp-CN possesses bactericidal properties against Streptococcus mutans. Keywords: Tooth decay, Antibacterial, silver nanoparticles, Streptococcus mutans, Clinacanthus nutans Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Miscellaneous Citation: Abd Ghafar S, Muhd Fudzi N, Wan Sulaiman W, Mohamad Hanafiah R and Lim V (2019). Antibacterial activity of silver nanoparticles Clinacanthus nutans (Agnp-CN) against Streptococcus mutans. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00062 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Siti Aisyah Abd Ghafar, Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Nilai, Malaysia, aisyahghafar@usim.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Siti Aisyah Abd Ghafar Nor Baitie Adura Muhd Fudzi Wan Nur Farhanah Wan Sulaiman Rohazila Mohamad Hanafiah Vuanghao Lim Google Siti Aisyah Abd Ghafar Nor Baitie Adura Muhd Fudzi Wan Nur Farhanah Wan Sulaiman Rohazila Mohamad Hanafiah Vuanghao Lim Google Scholar Siti Aisyah Abd Ghafar Nor Baitie Adura Muhd Fudzi Wan Nur Farhanah Wan Sulaiman Rohazila Mohamad Hanafiah Vuanghao Lim PubMed Siti Aisyah Abd Ghafar Nor Baitie Adura Muhd Fudzi Wan Nur Farhanah Wan Sulaiman Rohazila Mohamad Hanafiah Vuanghao Lim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.