Antibacterial activity of Nephrolepis biserrata extract against Aeromonas hydrophila and Vibrio parahaemolyticus

Abstract

Fish farming has become a significant sector in producing animal protein. However, a disease caused by bacteria such as Aeromonas hydrophila and Vibrio parahaemolyticus has become obstacle factors in these farming activities. Among the various procedures for preventing bacterial attacks, the use of natural ingredients deserves to be considered. This study aimed to test the ability of gray nail extract (Nephrolepis biserrata) in inhibiting the growth of A. hydrophila and V. parahaemolyticus in vitro. N. biserrata were extracted using methanol (EPM) and chloroform (EPC). EPM and EPC were then challenged with A. hydrophila and V. parahaemolyticus bacteria to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The test results showed that an inhibition zone of EPM against A. hydrophila and V. parahaemolyticus were 8.2 and 8.3 mm, respectively. EPC has been unable to inhibit the activity of both bacteria, whereas EPM could. The MIC value for both bacteria was 50 mg/mL, and MIB concentration was more than 50 mg/mL. The results of this study indicate that the N. biserrata extract could inhibit bacteria.