АНТИБАКТЕРИАЛЬНЫЕ И ФИЗИКО-ХИМИЧЕСКИЕ СВОЙСТВА НАНОЧАСТИЦ СЕРЕБРА И ОКСИДА ЦИНКА

Abstract

Background. Metal nanoparticles can have unique properties that differ from those of the solid metal from which they are obtained. This is what determines the increased interest in them on the part of researchers from all over the world. Objective of the study: quantitative assessment of the antibacterial effect of silver and zinc oxide nanoparticles on polyantibiotic-resistant strains of gram-positive and gram-negative microorganisms. Material and methods. The synthesis of silver and zinc oxide nanoparticles was performed by laser ablation in a liquid. The physical properties and sizes of these nanoparticles were studied on the basis of spectral characteristics and atomic force microscopy. The study of the antibacterial resistance of the studied microorganisms was carried out, as well as a quantitative assessment of the antibacterial effect of the obtained nanoparticles based on the determination of the minimum inhibitory and minimum bactericidal concentrations. Results. All microorganisms used in the study were clinical pathogenic strains with polyantibacterial resistance. In this case, the minimum inhibitory concentrations of silver nanoparticles ranged from 7.81 to 31.25 μg/ml, and the minimum bactericidal concentrations - from 31.25 to 62.50 μg/ml, while for zinc oxide nanoparticles the values of the minimum inhibitory concentrations were within the range from 125 to 500 μg/ml, and the values of the minimum bactericidal - from 250 to 1000 μg/ml. Conclusions. The laser ablation method allows the synthesis of chemically pure nanoparticles of silver, zinc oxide, and, if necessary, other metals. Silver and zinc oxide nanoparticles are effective antimicrobial agents against polyantibiotic-resistant pathogenic microbial flora. The obtained values of the minimum bactericidal and minimum inhibitory concentrations must be taken into account in the subsequent clinical implementation of medical devices based on the use of metal nanoparticles.