ANTIBACTERIAL ACTIVITY OF LAWSONIAINERMISAGAINST HUMAN PATHOGENIC BACTERIA

Abstract

Natural medicine has been the main source of primary healthcare all over the world. Since ancient times, plants have been used as a rich source of effective and safe medicines.Lawsoniainermishas efficacy on skin disease.Leaves of Lawsoniainermis provide an important cosmetic dye. Henna leaves were extensively used for centuries in the Middle East, the Far Eastand Northern Africa as dye for nails, hands, hair and textile.Lawsoniainermis contained 2-hydroxy-1,4-naphthoquinone (lawsone).Properties of Lawsoniainermis include antimicrobial, antifungal, antitumor, antiangiogenic, larvicidal, antileishmanial,antimalarial, hepatoprotective, anti-inflammatory, analgesic, and antipyretic characteristics.In this study the antibacterial activity of the leaf extract of Lawsoniainermison Escherichia coli, Staphylococcus aureus, andStreptococcus pyogenes. Zone of Inhibition was observed.Lawsoniainermisleaves extractcontaining test disk inhibited growth of pathogenic bacteria. Test disk containing 250 µg, 500 µg and 1000 µg showed 11mm, 14mm and 18 mm zone of inhibition against E. coli. 8 mm, 11mm and 13 mm zone of inhibition against Staphylococcus aureus. 7 mm, 9mm and 12 mm zone of inhibition against Streptococcuspyogenes. Whereas 30 µg Amikacin containing standard antibiotic disk showed 14 mm, 12 mm and 10 mm zone of inhibition against E. coli, S. aureus and S. pyogenes. This research concluded that Lawsoniainermisleaves extractcontain antimicrobial properties. The formulation of Lawsoniainermis leaves extract as a natural source for the development of drugs.