Antibacterial Activity of Four Plant Extracts Extracted from Traditional Chinese Medicinal Plants against Listeria monocytogenes, Escherichia coli, and Salmonella enterica subsp. enterica serovar Enteritidis.

R.L Mcmurray,N Corcionivoschi,M.E.E Ball,C Situ,M.M Tunney

doi:10.3390/microorganisms8060962

R.L Mcmurray, N Corcionivoschi + Show 3 more

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https://doi.org/10.3390/microorganisms8060962

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Abstract

The worldwide ethnobotanical use of four investigated plants indicates antibacterial properties. The aim of this study was to screen and determine significant antibacterial activity of four plant extracts in vitro and in a poultry digest model. Using broth microdilution, the concentrations at which four plant extracts inhibited Listeria monocytogenes, Salmonella enteritidis, and Escherichia coli over 24 h was determined. Agrimonia pilosa Ledeb, Iris domestica (L.) Goldblatt and Mabb, Anemone chinensis Bunge, and Smilax glabra Roxb all exhibited a minimum inhibitory concentration (MIC) of 62.5 mg/L and a minimum bactericidal concentration (MBC) of 500 mg/L against one pathogen. A. pilosa Ledeb was the most effective against L. monocytogenes and E. coli with the exception of S. enteritidis, for which A. chinensis Bunge was the most effective. Time–kills of A. pilosa Ledeb and A. chinensis Bunge against L. monocytogenes, E. coli and S. enteritidis incubated in poultry cecum were used to determine bactericidal activity of these plant extracts. A. chinensis Bunge, significantly reduced S. enteritidis by ≥ 99.99% within 6 h. A. pilosa Ledeb exhibited effective significant bactericidal activity within 4 h against L. monocytogenes and E. coli. This paper highlights the potential of these plant extracts to control pathogens commonly found in the poultry gastrointestinal tract.

Highlights

Antibiotic resistance is currently a global concern and significant research is taking place to tackle this issue [1,2], including research into the use of antibiotics in animal production [3]
The extracts of A. pilosa Ledeb, S. glabra Roxb, A. chinensis Bunge, and I. domestica (L.) Goldblatt and Mabb all demonstrated considerably low minimum inhibitory concentration (MIC) values ≤1000 mg/L and were chosen for further investigation to determine their effect on the inhibition and killing of L. monocytogenes, E. coli, and S. enteritidis in an in vitro digest model
This study demonstrates broad-spectrum antibacterial activity of four plant extracts, namely, A. pilosa Ledeb, S. glabra Roxb, A. chinensis Bunge, and I. domestica (L.) Goldblatt and Mabb against S. enteritidis, L. monocytogenes, and E. coli

Summary

Introduction

Antibiotic resistance is currently a global concern and significant research is taking place to tackle this issue [1,2], including research into the use of antibiotics in animal production [3]. In many countries outside of Europe antibiotics are used as poultry feed additives at subtherapeutic levels over prolonged periods to promote growth and control gastrointestinal infections in the flock [4,5]. While there are legitimate therapeutic reasons for antibiotic use in poultry farming there are concerns about the overuse and misuse of antibiotics [3]. A link between subtherapeutic application of broad-spectrum tetracyclines in agriculture and the development of acquired-resistant human isolates led to a ban on the use of tetracyclines for growth promotion in Europe in the early 1970s [6]. Alternatives to antibiotics could be used in livestock production to maintain production performance and control infections caused by pathogens commonly found in the poultry gastrointestinal tract. Escherichia, Salmonella, and Listeria [8,9,10]

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