Antibacterial activity of biosynthesized silver nanoparticles (AgNps) against Pectobacterium carotovorum.

Abstract

In a bioprospecting study of paramo soils cultivated with potato (Solanum tuberosum), 50 fungal isolates were obtained and evaluated for their nitrate reductase (NR) activity, given the role played by this enzyme in the biosynthesis of silver nanoparticles (AgNps). Five isolates strain with high NR activity belonging to Penicillium simplicissimum, Aspergillus niger, and Fusarium oxysporum species were selected, verifying the presence of the NR enzyme in their enzymatic extract. Later, these strains showed the ability to biosynthesize AgNps with distorted spherical shapes and sizes ranging from 15 to 45nm. Subsequently, an antibiosis test was carried out by the agar diffusion method using glass fiber disks against the phytopathogenic agent Pectobacterium carotovorum, finding halos of inhibition of bacterial growth up to 15.3mm using a 100ppm solution of the AgNps obtained from F. oxysporum. These results contribute to generating the basis of a new alternative for the control of this phytopathogenic agent of potato, challenging to manage by traditional methods and of relevance at the post-harvest level.