Anti-angiogenic effect of Ardisia crispa root hexane extract mediated via its angiogenic signalling cascades

Abstract

Event Abstract Back to Event Anti-angiogenic effect of Ardisia crispa root hexane extract mediated via its angiogenic signalling cascades Roslida Abd Hamid1*, Lim Wen Jun1 and Chan Pit Foong1 1 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia Background Angiogenesis, defined as the neovascularization from the pre-existing vasculature which involves the endothelial cells proliferation, migration and differentiation, is a vital process that occurs throughout different life stages. However, excessive angiogenesis leads to various diseases, including malignancy and non-neoplastic diseases. Ardisia crispa Thunb A.DC (Myrsinaceae), locally known as “hen’s eyes” in Malaysia, is a local medicinal plant with anti-inflammatory and anti-tumor promoting properties. This study aims to investigate the anti-angiogenic effect of the hexane extract of the plant’s root (ACRH) and its isolated benzoquinonoid (AC2) and its potential pathway(s). Methods Various angiogenesis assays in vitro against HUVECs, including zebra fish in vivo model were used in this study, followed by multiplex immunoassay in determining the pathway(s) involved. Results IC50 for ACRH and AC2 after 24 hours were reported to be 2.49 ± 0.036 and 1.35 ± 0.046µg/mL respectively. ACRH and AC2 showed significant inhibition in a concentration dependent manner (0.1, 1.0 and 10.0µg/mL) in comparison with the negative control (p<0.05) via cell migration, invasion and tube formation assays. In gelatin zymography, pro-MMP2 expression was reduced significantly in HUVEC after treated with ACRH and AC2 in a concentration dependent manner (p<0.05). Angiopoietin-2 (Ang-2), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF)-C and VEGF-D expression were reduced significantly (p<0.05) in a concentration dependent manner, whilst fibroblast growth factor (FGF)-1, FGF-2, Follistatin and heparin binding epidermal growth factor (HB-EGF) were significantly reduced at higher concentration upon ACRH and AC2 treatments, respectively via magnetic bead-based immunoassay. ACRH at 5ug/mL showed significant reduction of intersegmental vessels (ISV) sprouts whilst AC2 showed significant ISV development inhibition at 12.5ug/mL in zebra fish assay. Conclusion The present study evidenced the anti-angiogenic effect of ACRH and AC2 through both in vitro and in vivo assays which mediated via angiogenic signaling cascades. Acknowledgements We would like to thank Dr Okuda from Cancer Research Malaysia for providing the materials and facilities to perform zebra fish assay. Keywords: Ardisia crispa, Angiogenesis, HUVEC, cell invasion, zebra fish model Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: Abd Hamid R, Wen Jun L and Pit Foong C (2019). Anti-angiogenic effect of Ardisia crispa root hexane extract mediated via its angiogenic signalling cascades. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00055 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Roslida Abd Hamid, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia, roslida@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Roslida Abd Hamid Lim Wen Jun Chan Pit Foong Google Roslida Abd Hamid Lim Wen Jun Chan Pit Foong Google Scholar Roslida Abd Hamid Lim Wen Jun Chan Pit Foong PubMed Roslida Abd Hamid Lim Wen Jun Chan Pit Foong Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.