Synthetic cardamonin inhibits migration of serum-induced human bronchial smooth muscle cells (HBSMCs)

Abstract

Event Abstract Back to Event Synthetic cardamonin inhibits migration of serum-induced human bronchial smooth muscle cells (HBSMCs) Nazmi F. Musa1* 1 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia Background Increased smooth muscle mass is a prominent features of airways remodelling. This leads to persistent airflow limitation and impaired lung function which contribute to asthma. Inhaled corticosteroid, a primary treatment in asthma management, is unable to inhibit airway remodelling. Hence, effective treatments targeting airway remodelling in asthma are urgently needed. Increased smooth muscle mass may be attributed to both the proliferation and migration of smooth muscle cells. Cardamonin is a chalcone analog that has been reported to inhibit migration of vascular smooth muscle cells. However, its effects upon the migratory capability of bronchial smooth muscle cells have yet to be determined. The ability of synthetic cardamonin to suppress serum-induced Human Bronchial Smooth Muscle Cells (HBSMCs) migration and the mechanisms involved were therefore be evaluated. Methods Briefly, HBSMCs were treated with the cardamonin (0.75-12.5uM) in a complete media for 48 hours. The effects of cardamonin on HBSMCs migration were assessed through scratch and transwell assays. Meanwhile the effects of cardamonin (12.5uM) on the expression of proteins associated with cell migration (RhoA and phospho-cofillin) were determined by western blot. Results The scratch and transwell assays demonstrated that synthetic cardamonin (0.78-12.5uM) reduced the HBSMCs migration in a dose-dependent manner. The inhibitory effect of cardamonin on migration of serum-induced HBSMCs is associated with a reduced expression of RhoA and increased phosphorylation of cofillin. Conclusion Synthetic cardamonin is able to reduce the migration of serum-induced HBSMCs via downregulation of RhoA and upregulation of phosphorylation of cofillin. Acknowledgements Daud Ahmad Israf Ali, Tham Chau Ling, Hanis Hazeera Harith, Manraj Singh Cheema Keywords: Airway remodeling in asthma, Cardamonin (PubChem CID: 10424762), airways smooth muscle migration, serum-induced Human Bronchial Smooth Muscle Cells, Actin cytoskeletal remodeling Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Inflammatory diseases Citation: Musa NF (2019). Synthetic cardamonin inhibits migration of serum-induced human bronchial smooth muscle cells (HBSMCs). Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Mr. Nazmi F Musa, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia, nazmi908991@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nazmi F Musa Google Nazmi F Musa Google Scholar Nazmi F Musa PubMed Nazmi F Musa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.