Antiangiogenic Activity of Oregano (Origanum Vulgare) Aqueous Extract In-Vivo Through Chick Chorioallantoic Membrane (Cam) Assay

Abstract

Angiogenesis is the formation of new blood vessels and is required in tumor growth, metastasis and cancer progression. Philippine traditional medicine preparations have shown a comprehensive and function- regulating characteristics. Oregano (Origanum vulgare) is an annual succulent herb which distributed throughout the world. Currently, there have been some related reports on the preventive effects on chronic diseases such as cancer and heart diseases with oregano. The study aimed to evaluate the antiangiogenic activity of oregano crude leaf extract through Chick Chorioallantoic Membrane (CAM) assay. The density and length (per branching) of the blood vessels were measured through a microscope and ToupView software calibrated using a micrometre respectively. The researchers analyzed the data gathered using one-way Analysis of Variance (ANOVA), post hoc tests were also performed using Duncan Multiple Range Test (DMRT) to confirm the results of the ANOVA. Statistical results in the mean blood vessel density and length of blood vessels among the treatments which is at .001 and .000 respectively is lesser than the 0.05 level of significance which rejects the null hypothesis. DMRT results confirm the significant differences among the treatments. The results indicate that the crude leaf extract of Origanum vulgare significantly reduced the length and density of the blood vessels of chick chorioallantoic membrane, indicating its angiogenesis inhibiting capabilities. These results suggest that antiangiogenic effects of crude leaf extract of Origanum vulgare may be mediated via its antioxidant and anticancer effects.