Abstract
Research on antioxidants has been gaining worldwide attention because of their essential applications for medicinal purposes. In this study, we conducted bioprospecting of six Asteraceae plants as the source of antiaging and antioxidant agents. Water and chloroform fractions from Ageratum conyzoides L., Dichrocephala integrifolia (L.f.) Kuntze, Galinsoga parviflora (Cav.), Mikania micrantha Kunth, Sphagneticola trilobata (L.) Pruski, and Synedrella nodiflora L. were collected and assayed for their in vitro antioxidant activities and potential antiaging properties using the yeast Schizosaccharomyces pombe as the model organism. Based on the in vitro assay, the water fractions of S. trilobata showed a strong antioxidant activity. Interestingly, all treatment solutions promoted the stress tolerance phenotype of S. pombe to strong H2O2-induced oxidative stress conditions. Moreover, compared with the treatments without plant extract/fraction, all extract and fraction treatments, except the chloroform fractions of A. conyzoides, promoted yeast cell longevity. Strong induction of mitochondria activity was found following the treatments with the extracts and fractions of S. nodiflora, D. integrifolia, and M. micrantha and likely mimicked the calorie restriction-induced lifespan. Interestingly, S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. These data indicated that the fractions of Asteraceae plants had potential antioxidant and antiaging activities through various cellular modulations. S. nodiflora water fraction has been shown to have antioxidant and antiaging activities in S. pombe, by modulating stress tolerance response, inducing mitochondrial activity, and increasing the ctt1 gene expression. Compounds analysis identified that S. nodiflora water fraction contained some primarily compounds including oxyphyllacinol, valine, and sugiol.
Highlights
Free radical accumulation has been known to induce oxidative stress conditions, which further damage cellular functions [1, 2]. rough the activation of enzymatic and nonenzymatic antioxidants, the human body has complex defense systems against these damaging effects of free radicals [3, 4]
S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. ese data indicated that the fractions of Asteraceae plants had potential antioxidant and antiaging activities through various cellular modulations
Exogenous antioxidants, which are Advances in Pharmacological and Pharmaceutical Sciences known as dietary antioxidants, are often used to reduce the damaging effects of free radicals. erefore, the intake of exogenous antioxidants may compensate for the inadequacy of cellular antioxidant activity in combating oxidative stress conditions [6]
Summary
Free radical accumulation has been known to induce oxidative stress conditions, which further damage cellular functions [1, 2]. rough the activation of enzymatic and nonenzymatic antioxidants, the human body has complex defense systems against these damaging effects of free radicals [3, 4]. Free radical accumulation has been known to induce oxidative stress conditions, which further damage cellular functions [1, 2]. As the body ages or after intensive exposure to exogenous free radical sources, the cellular defense systems may be compromised and cause severe oxidative stress conditions, which often culminate in the development of degenerative diseases [5]. Until today, analyses of the antioxidant activities of Asteraceae-derived extracts in modulating cellular systems have been very limited. Such information is essential for the comprehensive evaluation of the antioxidant properties of the extracts, especially in revealing the mechanism of actions at the cellular level, and the application of these extracts as dietary antioxidants for daily use
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
