Anti-adhesive and anti-biofilm activity of NC-E08. In vitro and in vivo study using Galleria mellonella infection model

Eder R Paganini,Gabriela Fernanda Bombarda,Janaina De Cássia Orlandi Sardi,Josy G Lazarini,Luis O Regasini,Pedro L Rosalen

doi:10.20396/revpibic262018506

Abstract

Biofilms are organized microbial communities formed from an ecological succession. Biofilm formation functions as a mechanism of virulence and favors the development of diseases, including oral diseases such as dental caries and periodontal disease, in which the microorganisms Streptococcus mutans and Candida albicans are closely related. Previous studies have shown that interactions between S. mutans and C. albicans are associated with the pathogenesis of early childhood caries (ECC). Therefore, there is a great interest in finding new prototypes for antimicrobial drugs, mainly for the development of structural analogues of chalcones, which constitute one of the largest classes of natural products belonging to the flavonoid family and are considered strategic molecules for this purpose.

Highlights

Biofilms are associated with many infectious diseases in humans, including those occurring in the mouth
Previous studies have shown that interactions between S. mutans and C. albicans are associated with the pathogenesis of early childhood caries (ECC)
Biofilms mixed are considered resistant to conventional therapies and this fact has stimulated the search for alternative antimicrobial agents that can prevent biofilms formation and the onset of associated diseases. The objective of this project was to evaluate the effect of the nitrochalcone (NC-E08) on mixed biofilms of C. albicans and S. mutans formed on hydroxyapatite disks, through cell viability, Microscopy Scanning Electron (MEV), in vivo and in vitro adhesion and Galleria mellonella model infection

Summary

Introduction

Biofilms are associated with many infectious diseases in humans, including those occurring in the mouth. In vitro and in vivo study using Galleria mellonella infection model Biofilm formation functions as a mechanism of virulence and favors the development of diseases, including oral diseases such as dental caries and periodontal disease, in which the microorganisms Streptococcus mutans and Candida albicans are closely related.

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Results

Conclusion