Anti-Yeast Synergistic Effects and Mode of Action of Australian Native Plant Essential Oils

Fahad Alderees,Stephen Were,Ram Mereddy,Michael E Netzel,Yasmina Sultanbawa

doi:10.3390/app112210670

Abstract

Yeasts are the most common group of microorganisms responsible for spoilage of soft drinks and fruit juices due to their ability to withstand juice acidity and pasteurization temperatures and resist the action of weak-acid preservatives. Food industries are interested in the application of natural antimicrobial compounds as an alternative solution to the spoilage problem. This study attempts to investigate the effectiveness of three Australian native plant essential oils (EOs) Tasmanian pepper leaf (TPL), lemon myrtle (LM) and anise myrtle (AM) against weak-acid resistant yeasts, to identify their major bioactive compounds and to elucidate their anti-yeast mode of action. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum bactericidal concentration (MBC) were assessed for EOs against weak-acid resistant yeasts (Candida albicans, Candida krusei, Dekkera anomala, Dekkera bruxellensis, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Zygosaccharomyces bailii and Zygosaccharomyces rouxii) and bacteria (Staphylococcus aureus and Escherichia coli). The EOs showed anti-yeast and antibacterial activity at concentrations ranging from 0.03–0.07 mg/mL and 0.22–0.42 mg/mL for TPL and 0.07–0.31 mg/mL and 0.83–1.67 mg/mL for LM, respectively. The EOs main bioactive compounds were identified as polygodial in TPL, citral (neral and geranial) in LM and anethole in AM. No changes in the MICs of the EOs were observed in the sorbitol osmotic protection assay but were found to be increased in the ergosterol binding assay after the addition of exogenous ergosterol. Damaging of the yeast cell membrane, channel formation, cell organelles and ion leakage could be identified as the mode of action of TPL and LM EOs. The studied Australian native plant EOs showed potential as natural antimicrobials that could be used in the beverage and food industry against the spoilage causing yeasts.

Highlights

Plant essential oils (EOs) have been utilized for many centuries in traditional medicine, as spices, coloring, perfume and aromatherapy [1,2]
EOs of lemon myrtle (LM) and anise myrtle (AM) were supplied by Australian Rainforest Products Pty Ltd. (Lismore, NSW, Australia) and EO of Tasmanian pepper leaf (TPL) was supplied by Essential Oils of Tasmania Pty Ltd. (Margate, TAS, Australia)
TPL and LM EOs demonstrated a broad-spectrum of weak-acid resistant anti-yeast and antibacterial activity

Summary

Introduction

Plant essential oils (EOs) have been utilized for many centuries in traditional medicine, as spices, coloring, perfume and aromatherapy [1,2]. Due to their beneficial properties, they have been incorporated in recent years into pharmaceuticals, food preservation, agriculture, disinfection and sanitation products, and cosmetics [3]. There are about 3000 known EOs; only 10% have been commercially utilized in different industries [4,5]. There is a huge opportunity to study and exploit the biological properties of these EOs for novel applications in the food (e.g., as natural preservatives) and pharmaceutical industries. Little is known about their efficacy when compared to synthetic food preservatives, their activity against weak-acid resistant yeasts and mode of action [6,7,8,9,10]

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Conclusion