Anti-sense RNA inhibits the expression of Ezrin and invasion of breast cancer cells

Abstract

Objective To observe the influence of proliferation and invasion of human breast cancer ceils MDA-MB-231 after inhibition of Ezrin expression by anti-sense RNA. Methods Human breast cancer cell lines MDA-MB-231 and MCF-7 were transfected with anti-pCR3, 1-Ezrin with lipo-fectamine. Western-blot and RT-PCR were used to detect the expression of Ezrin after transfection for 6, 12,and 24 h,respectively. The proliferative ability of ceils was tested by MTT assay,and the metastatic a-bility of cells was investigated by Boyden cabin after transfection for 24 h. Results After transfection with anti-sense RNA for 24 h,both protein and mRNA expression of Ezrin in MDA-MB-231 cells was signifi-cantly reduced at their peak time. After transfection with anti-sense RNA for 24 h, the MTT assay showed that absorbance (A) values in anti-pCR3, 1-Ezrin,vacuity plasmid and blank control groups were 0.410±0.018,0.765±0.058,0.795±0.061 in MDA-MB-231, and 0.480±0.021,0.632±0.052,0.648±0.059 in MCF-7, respectively. The results suggested that the proliferation of MDA-MB-231 and MCF-7 cells was inhibited significantly in anti-pCR3.1-Ezrin group by (47.9±3.1) % and (32.0±2.8) %, re-spectively. After transfection with anti-sense RNA for 24 h, the invasive ability of MDA-MB-231 and MCF-7 cells was (50.5±3.2) % and (74.8±4.6) % respectively as compared with that of blank control group (P<0.05). Conclusion Ezrin plays an important role in the process of proliferation and invasion of breast cancer cells. Key words: Breast neoplasm; Antisense RNA; Invasion; Ezrin