Abstract
Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity.
Highlights
Dolichos lablab L. (DLL), known as hyacinth bean and part of the family Fabaceae, is widely grown in Africa and southern Asia, including in India and China [1]
The results showed that the isolated triterpenoid saponin, Chikusetsusaponin IVa (CS-IVa), and DLL-Ex inhibited the differentiation and maturation of 3T3-L1 cells by inhibiting the expression of the adipogenic transcription factors PPARγ and C/EBPα
These results suggest that CS-IVa and DLL-Ex might be developed into a drug for the control nutraceutical treatment of obesity and related diseases
Summary
Dolichos lablab L. (DLL), known as hyacinth bean and part of the family Fabaceae, is widely grown in Africa and southern Asia, including in India and China [1]. DLL seeds are used for food and as a medicinal plant; it is reported that DLL is an effective agent against hypercholesterolemia, poison, gastrointestinal spasms, cholera, vomiting, diarrhea, leucorrhoea, and alcoholic intoxication [2,3,4]. Previous studies have reported the isolation of chikusetsusaponin (CS)-IVa and lablabosides A, B, C, D, E, and F from DLL seeds [11]. The purification of flavonoids, including cosmosiin, luteolin, luteolin-40 -O-β-D-glucopyranoside, luteolin-7-O-β-D-glucopyranoside, and rhoifolin from DLL flowers has been reported [12]. Obesity is a condition where excess body fat accumulates to the extent that it causes a negative effect on health. Obesity increases the risk of obstructive sleep apnea, type 2 diabetes, Nutrients 2018, 10, 1221; doi:10.3390/nu10091221 www.mdpi.com/journal/nutrients
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
