Abstract
An antimicrobial agent, chloramphenicol, was isolated from Actinobacteria 10CM9. The strain was isolated from lake sediment collected from Erçek Lake, Van (38°29’57.76“N and 43°33’10.98”E) and AIA medium was used as a isolation medium. 16S rRNA gene sequence analysis that showed the strain belongs to the genus Streptomycetes. A fermentation study in AIA broth medium (3L) followed by extraction with EtOAc afforded 105 mg of crude extract. A bioactivity-guided fractionation by Open Column chromatography on silica gel led to the isolation of one secondary metabolite (10CM19-01, 31 mg). Structures of the metabolites were established as chloramphenicol, by means of NMR (1H, 13C and COSY) data. Antimicrobial activity of 10CM9 was tested by microdilution method against enteropathogenic Escherichia coli 0157:H7 (RSKK 234), methicillin resistant Staphylococcus aureus ATCC 43300, vancomycin resistant Enterococcus faecium DSMZ 13590, Candida albicansDSMZ 5817 and Pseudomonas aeruginosa ATCC 27853. As a result, 0.5 μg/ml, 0.5 μg/ml and 1 μg/ml MIC values were observed against Escherichia coli 0157:H7, Enterococcus faecium and Staphylococcus aureus, respectively.
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