Anti-Liver Cancer Activity of a New Compound Extracted from Populus pruinosa and Phylogenetic Analysis

Abstract

Populus pruinosa is a fragile poplar species separated from other poplar trees. It is specifically adapted to tropical areas and utilized as a conventional medicine. It is an important medicinal plant for the treatment of cancer. A new compound was extracted from P. pruinosa. Its application value for the treatment of liver cancer was then explored. The corresponding mechanism was also determined. After treatment with this compound, CCK-8 was detected and the proliferation of liver cancer cells was examined. Furthermore, the percentage of hepatocyte apoptosis was calculated via Annexin V-FITC/PI apoptosis analysis. The total sequence of the chloroplast genome of P. pruinosa was determined by terminal sequencing with Illumina. Neighbor-joining phylogenetic analysis suggested that P. cathayana and P. pruinosa cluster together as the sister groups to other poplar species.