Anti-inflammatory Test of Centella Asiatica Extract on Rat Induced by Cadmium

Hernayanti Hernayanti,Sri Lestari,Saryono Saryono,Puji Lestari

doi:10.20884/1.jm.2021.16.3.775

Abstract

Cadmium is a toxic heavy metal. The present of cadmium caused inflammation in liver. This study aims to know the anti inflammatory of Centella asiatica extract on rat induced by cadmium. This research was an experimental study using post test only control group design. Twenty four rats divided into six groups with four replications, i.e group of healthy control (C1), negative control induced by CdSO4 with dosage 56 mg/kg for 14 days and treatment (C3-C6) with dosage of 100, 200 ,300 and 400 mg/kg of C. asiatica. The blood Cd, GST, GSH, TNF- α and COx2 were measured after a 21 days administration of C. asiatica. The data were analyzed by ANOVA test followed by Duncan test with a significance level of 5%. The result showed that administrating C.asiatica can neutralized cadmium, improve inflammation in liver.The conclusion of our research that C.asiatica extract can decrease Cd level, TNF-α and COx2 levels and increase GST and GSH level in rat induced by cadmium. A 200 mg/kg was the effective dosage to reduce Cd, TNF-α and COx2 levels and increase GST and GSH levels. Key words : anti inflammatory, Cd, Centella asiatica, GST, COX2

Highlights

The heavy metal cadmium (Cd) is a dangerous compound since it can pollute the environment and cause health problems for humans
One clinical study showed that environmental Cd exposure was either related with hepatic necroinflammation, non-alcoholic fatty liver disease (NAFLD), and non-alcoholic steato hepatitis (NASH) in men or hepatic necro-inflammation in women (Choi et al, 2016)
Data analysis among the healthy control, positive control and treated groups were significantly different. These data show that Cadmium enters the rats body and binds to metalothionein and further it generates oxidative stress which occurs when the generation of free radicals or reactive oxygen species (ROS) such as 1O2, O2- and OH- exceed the antioxidant capacity of a biological system (Bernhoft, 2013., Prabu & Bashir, 2014)

Summary

Introduction

The heavy metal cadmium (Cd) is a dangerous compound since it can pollute the environment and cause health problems for humans. Anti inflammatory test of Centella asiatica extract vegetables, fruits and medicinal plants have been explored in many laboratories; and becoming candidates in preventing cells from oxidative stress, and simultaneously promoting the costumer’s health due to its phenolic compounds. These two compounds of asiaticoside and asiatic acid have been reported to have protective effects against kidney injury following an induced sepsis, more likely associated with the inhibition of IL-6 and TNF α in serum and the inducible Nitric Oxide Synthase (iNOS) enzyme in kidney tissues.

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