Anti-inflammatory quinolizidine alkaloids from the aerial parts of Sophora tonkinensis

Abstract

• Seventeen alkaloids were isolated from the aerial parts of Sophora tonkinensis. • All isolates were evaluated for their inhibitory effects on the NO production in LPS-induced RAW 264.7 macrophages. • Compounds 3 , 4 , 13 , and 14 were shown to have moderate anti-inflammatory effects. Seventeen quinolizidine alkaloids, including two new ones, 5-hydoxy-7,11-dehydromatrine ( 1 ) and 11-hydroxylupanine ( 2 ), were isolated from the aerial parts of Sophora tonkinensis. The structures of the isolates were elucidated using 1D and 2D NMR analyses, as well as HR-ESI-MS spectroscopic data. The anti-inflammatory activities of all the isolates on NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) were evaluated. Compounds 3 , 4 , 13 , and 14 were shown to have moderate anti-inflammatory effects.