Anti-Inflammatory Effects of Weigela subsessilis Callus Extract via Suppression of MAPK and NF-κB Signaling

Hyeon-Ji Lim,Suk Weon Kim,Eun Yee Jie,Woo Seok Ahn,Chan-Hun Jung,Sang-Jun Kim,Seung-Il Jeong,In-Sun Park

doi:10.3390/plants10081635

Abstract

Weigela subsessilis is used in folk medicine to treat pain and allergic syndromes in Korea. However, the antibacterial and anti-inflammatory activities of W. subsessilis callus extract remain unexplored. In this study, we aimed to evaluate the W. subsessilis callus of pharmacological activity. Therefore, we first established in vitro calluses of W. subsessilis via plant tissue culture methods. We then evaluated the antioxidant and anti-inflammatory effects of W. subsessilis callus extract in lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells. The W. subsessilis callus extract showed antioxidant and anti-inflammatory effects. These effects were regulated via suppression of mitogen-activated protein kinase signaling through LPS-induced translocation of nuclear factor kappa B (NF-κB) p65 from the cytoplasm to the nucleus. W. subsessilis callus extract also showed antibacterial and anti-inflammatory activities in Propionibacterium acnes-treated HaCaT keratinocyte cells. These results indicate that W. subsessilis callus extract has antioxidant, antibacterial and anti-inflammatory activities, suggesting its possible application in the treatment of inflammatory disorders.

Highlights

IntroductionW. subsessilis (WS) has been cultivated in Korea and Japan
We measured the protein levels of inducible NO synthase (iNOS) and COX-2 via western blotting assay blotting assay (Figure 3f). These findings suggested that WS callus (WSC) extract inhibits Nitric oxide (NO) and
These findings suggested that WSC extract inhibits NO and PGE2 production by suppressing the mRNA and protein levels of iNOS and COX-2

Summary

Introduction

W. subsessilis (WS) has been cultivated in Korea and Japan. It is used in folk medicine to treat pain and allergic syndromes in Korea. WS has various pharmacological activities, including anti-complement activation effect, stimulation of melanogenesis, inhibition of low-density lipoprotein oxidation, stimulation of glucose uptake and anti-inflammatory activity [1,2,3,4]. Studies on the chemical constituents of WS have shown the presence of flavonoids, coumarins, terpenoids, sterols and iridoids [5]. Among the chemical constituents of WS, coumarins, terpenoids and iridoids have an broad spectrum of pharmacological functions, such as anti-cancer, anti-inflammatory and antibacterial activities [6,7,8,9]. Other chemical constituents of WS, including flavonoids and sterols, exhibit antioxidant activities [10,11,12]

Objectives

Methods

Discussion

Conclusion