Abstract
To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.
Highlights
In recent years, many metabolites from marine organisms with significant potential anti-inflammatory activities have been discovered
Comaparvin (1 μM) did not significantly affect the viability of macrophage cells at 24, 48, and 72 h, but the viability of the cells was significantly lower when comaparvin was used at 50 μM for 72 h and at 100 μM for 48 and 72 h, as compared with the vehicle (DMSO) groups
The viability of macrophage cells treated with comaparvin at 5, 10, and 25 μM
Summary
Many metabolites from marine organisms with significant potential anti-inflammatory activities have been discovered. Several new medications based on substances from these marine organisms have undergone clinical and preclinical trials [1]. Natural ingredients obtained from the ocean, especially from marine invertebrates, have become important sources of new compounds [2,3,4]. Many studies have screened the anti-inflammatory properties and activities of marine compounds for the treatment of chronic illnesses [1,4,5]. In 1967, Bolker extracted and isolated a steroid compound, crinosterol, from comatulid crinoids [6]. In 1971, Smith and Sutherland isolated several quinone compounds and naphthopyrones from
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