ANTI-INFLAMMATORY ACTIVITY OF GINGER (Zingiber officinale) THICK EXTRACT ON THE MODEL OF TRYPSIN-INDUCED INFLAMMATION

Abstract

Introduction. The plant-origin screening for highly-active anti-inflammatory compounds is one of issues of the day for modern pharmacology. Ginger rhizomes ( Zingeber officinale ) is a potentially perspective source for it. Aim. To study concentration-dependent anti-inflammatory effect of ginger extract-containing ointment on the model of trypsin-induced inflammation under conditions of prophylaxis (2 days before) and treatment (after inducing inflammation) schemes. Methods. Inflammation was induced by subplanary injected trypsin (0.1 ml, 0.5% solution). Anti-inflammatory action was estimated as foot edema inhibition in comparison with both initial values (before flogogen injection) and those of the control group. In addition to morphological signs, hematological indicators were determined (total leukocytes and leukogram – white blood count) before the experiment and 3, 6 and 24 hours after inducing inflammation. Results. Pharmacological screening of ginger extract-containing ointments (0.0125; 0.025; 0.05 %) on the trypsin-induced foot inflammation model showed that increase of concentration from 0.0125 % to 0.025 % progressively increased the anti-inflammation activity of the studied samples. However, further increase of the extract content in ointment samples (0.05 %) did not lead to substantial increase of anti-inflammatory action, but on the contrary even aggravated inflammation process, which permits to consider the concentration of 0.025 % the most effective for studying anti-exudative activity on other inflammation models. Conclusions. The conducted studies showed that the most effective anti-exudative effect on the trypsin-induced inflammation was demonstrated by 0.025 % ginger extract-containing ointment in comparison with other concentrations.