Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis.

Lijia Zhang,Xi Feng,Salam A Ibrahim,Wen Huang,Ying Liu,Mengzhou Yin

doi:10.3390/foods10123155

Abstract

In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

Highlights

Poria cocos (Schw.) Wolf (“fuling” in Chinese) is a widely used medicinal fungus.According to the Chinese Pharmacopoeia, about one-tenth of traditional Chinese medicine preparations include P. cocos [1]
Four triterpenoids were isolated from Poriae Cutis: two 3,4-seco-lanostan7,9(11)-diene type triterpenes and two lanosta-7,9(11)diene type triterpenes
The 3,4-seco-lanostan-7,9(11)-diene type triterpenes were found to inhibit the Nitric oxide (NO) generation in LPS-induced RAW264.7 cells

Summary

Introduction

Poria cocos (Schw.) Wolf (“fuling” in Chinese) is a widely used medicinal fungus.According to the Chinese Pharmacopoeia, about one-tenth of traditional Chinese medicine preparations include P. cocos [1]. P. cocos is used as a dietary supplement and in foods such as soups, dishes, snacks, and desserts for its health-promoting benefits. It can be divided into four parts, namely, Poriae Cutis (the epidermis, “fulingpi” in Chinese), Rubra Poria (the pink part near the epidermis), White Poria (the middle part, “baifuling” in Chinese), and Poria cum Radix Pini (the middle-plus-inner part, “fushen” in Chinese) [2]. Different parts of Poria cocos have different pharmacological effects and clinical applications. Both P. cocos and Poriae Cutis are officially included in the Chinese Pharmacopoeia.

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