Anti-Hepatoma Activity of A New Selenium-Rich Chitosan from 6-Hydroxy Esterification of Chitosan Copper

Abstract

This paper presents a technique to produce a selenium-rich chitosan by 6-hydroxy esterification of chitosan copper whose mole ratio of chitosan monomer and Cu is almost 1:1, which is suitable for the fields of food and feed chemistry or fine chemical technology, and belongs to the field of biomedical engineering technology. 6-Selenite chitosan copper will have better application prospect in medicine and make as selenium nutrient supplement in food, feed and agricultural fields, due to its higher selenium content and lower dosage than existing seleno-chitosan. The selenite chitosan copper effect than the common selenate chitosan. Selenite chitosan copper, which contains 10 times more selenium then existing seleno-chitosan or selenate chitosan, can greatly reduce its use and have a better anti-hepatoma effect. Metal ion protected amino group can bind to acid in the process of biological metabolism, and has a good targeting effect on the weak acidic environment of hepatocellular carcinoma tumors. Previous experiments in vitro showed that the IC50 value of selenite chitosan copper inhibiting HepG2 cells was 20.3 mg/L, and the inhibition rate to HepG2 cells was 87% at the concentration of 50 mg/L.