Abstract
Background:Glioblastoma is the most common primary malignant tumor of the central nervous system that occurs in the spinal cord or brain. Pseudosynanceia Melanostigma is a venomous stonefish in the Persian Gulf, which our knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondria isolated from U87 cells. Methods:In the first stage, we extracted venom stonefish and then isolated mitochondria have exposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase (SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP), and mitochondrial swelling) have evaluated. Results:To determine mitochondrial parameters, we used 115, 230, and 460 µg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstream parameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion:This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma.
Highlights
Glioblastoma multiform is the most common group of brain tumors and malignant gliomas are the deadliest types of adult brain tumors
This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals
The goal of this study was to evaluate the toxic effects of P. melanostigma venom using mitochondria isolated from U87 cells
Summary
Glioblastoma multiform is the most common group of brain tumors and malignant gliomas are the deadliest types of adult brain tumors. Glioblastoma multiform cells (U87) are very different in shape and size; they have high vascular proliferation and necrosis and increase the involvement of tumor cells in different areas of the brain. This type of tumor has a poor prognosis with various clinical features such as cognitive impairment, personality changes, headache, loss of sensation (paresthesia), and gait imbalance. Conclusion: This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma
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