Anti-diabetic Potential of Aqueous Extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina in Alloxan-Induced Diabetic Rats

A L Adesiyan ,K A Oladimeji ,E A Kade ,Kehinde Sowunmi ,K Gurpreet

doi:10.33696/diabetes.1.023

Abstract

The incidence of diabetes mellitus (DM) is increasing globally, and it is a major source of concern. This study was undertaken to assess the antidiabetic effect of the aqueous extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina. Sixty adult Wistar rats with body weight of 120-150 g were randomly assigned to groups of five rats each (n=12). Group 1 served as normal control; Groups 2-5 were diabetic groups; group 2 served as negative control; group 3-5 received 100, 200 and 400 mg/kg of triherbal formula, respectively. The body weight (BW) and fasting blood glucose level (FBSL) of the rats were monitored weekly. At the end of the experiment, all the rats were anaesthetized intraperitoneally (I.P) and blood samples were collected by cardiac puncture for biochemical analysis. There was an increase in the BW of the control group and varying doses of tri-herbal formulation. It caused 88?crease in FBSL; 371.7%, 386.6% and 296.0% with respect to 100, 200 and 400 mg/kg. Sub-chronic study of the effect of the extract showed a significant increase (P<0.05) in packed cell volume (PCV), white blood counts in rat induced diabetes. The histological studies showed that the diabetic rats with the architecture of the pancreas distorted, was restored to normal by the extract. Its LD50 was found to be greater than 1000 mg/kg indicating its safety in rats. This study has shown that triherbal formula has hypoglycemic and hematological effects.

Highlights

Diabetes mellitus (DM) is characterized by absolute or relative deficiencies in insulin secretion and insulin action associated with chronic hyperglycemia and disturbances of carbohydrate, lipid and protein metabolism [1,2,3]
The extract showed a significant reduction (P
Anti-diabetic potential of aqueous extract of Moringa oleifera, Ocimum gratissimum, and Vernonia amygdalina in alloxan-induced diabetic rats showed that a single oral administration of the extract reduced the fasting blood glucose levels as well as suppressed the rise in blood glucose of normal rats after a heavy glucose meal

Summary

Introduction

Diabetes mellitus (DM) is characterized by absolute or relative deficiencies in insulin secretion and insulin action associated with chronic hyperglycemia and disturbances of carbohydrate, lipid and protein metabolism [1,2,3]. It results from either defect in insulin secretion by the pancreas or inability of the cells to utilize the insulin produced [4,5]. DM is a global burden, associated with life-threatening complications including stroke, renal failure, and cardiac attack. 1 in 10 adults worldwide are affected with diabetes [6,7,8]

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