Anti-cancer Properties of Protein Hydrolysate from the Posterior Salivary Gland of Amphioctopus membranaceus (Quoy & Gaimard, 1832)

Abstract

The present study was carried out to evaluate the anti-cancer properties of protein hydrolysate from posterior salivary gland (PSG) of Amphioctopus membranaceus. The sample (octopus) was initially screened for species identification through CO-I gene. The PSG of the animal was homogenized and centrifuged at 6000 rpm for 10 min. Further, enzymatic hydrolysis was performed using papain enzyme at a dose of 2.5% w/w. Later the hydrolysate (supernatant) obtained was subjected to fractionation using gel permeation chromatography. 20 fractions were collected and the fraction 4 (OM-H) was found to have the maximum amount of protein content of 3.2 mg/mL. SDS-PAGE depicted prominent bands ranging from 14 to 25 kDa. The IC50 evaluation was studied using Vero cell lines that showed the cytotoxic effect of OM-H at 405 μg/mL and in HeLa cell lines 250 µg/mL showed the highest inhibition rate of 45.01%. Thus, the study showed that partially purified PSG hydrolysate was found to display good anti-cancer activity against human cancer cell lines.