Anti-biofilm and antioxidant activities of Sargassum muticum extracts and their preservation effect of Chinese shrimp (Penaeus chinensis)

Abstract

Vibrio parahaemolyticus can enhance bacterial resistance to disinfectants and antimicrobials by forming biofilms. The development of new natural antimicrobials is essential for the control of biofilm-related contamination and infection. In this paper, we preliminarily explored the inhibitory activity of Sargassum muticum extracts (HA-PTs) against the biofilm of V. parahaemolyticus. HA-PTs exerted anti-biofilm activity by destroying the integrity of the cell membrane, curbing the motility of single and colonial bacteria, hindering the synthesis of polysaccharide intercellular adhesion, inhibiting the secretion of extracellular DNA, and reducing the metabolic activity of biofilms. Furthermore, experiments on four free radicals (DPPH•, •OH, ABTS+•, and O2–•) scavenging activity and total antioxidant capacity (FRAP method and phenanthroline method) demonstrated that HA-PTs have strong reducing properties and can capture a variety of free radicals, which in turn prevents them from participating in oxidative reactions. When HA-PTs were applied to Chinese shrimp infected with V. parahaemolyticus, it could prolong their shelf life by controlling the pH rise caused by protein degradation, inhibiting the bacterial proliferation, reducing the content of total volatile basic nitrogen produced by protein degradation, and reducing the accumulation of thiobarbiturates caused by oxidative deterioration of fats.