Anti-bacterial activity of Dayak onions extract (Eleutherine palmifolia) against Salmonella spp and Escherichia coli

Abstract

Dayak onions (Eleutherine palmifolia) is a plant from Kalimantan. Dayak onions contain high flavonoid and phenol compounds and can be used as an alternative feed additive for broilers to replace synthetic antibiotics that can cause resistance and residues. This study aims to determine how much the ability of Dayak onions extract to inhibit Salmonella spp and Escherichia coli bacteria by using factorial randomized design and Duncan’s advanced test with three replications. The first factor was the maceration test consisting of control using tetracycline and replication of maceration I, maceration II, combined maceration I and II. The second factor is the concentration consisting of three concentrations of 0.50%, 1.00% and 1.50%. Antimicrobial activity is characterized by the presence of a clear zone that forms around a good hole. The results showed that the best antimicrobial activity of Dayak onion extract was obtained in K3M1 with inhibitory zone diameters of 15.47 mm for Salmonella spp and 13.40 mm for Escherichia coli. The value of the diameter of the inhibition zone shows a high sensitivity level as an antibacterial. The lowest diameter value of the inhibition zone was K1M2 which was 3.40 mm for Salmonella spp and 4.18 mm for Escherichia coli. It was concluded that the best replication factor of the antibacterial activity was at M1 and the higher the concentration of Dayak onion extract was used, the higher the antibacterial activity and could be used as an alternative feed additive.