Abstract
Dayak onions ( E. palmifolia (L) Merr) is a herb used as medicine and known to have antibacterial activity. This research aimed to evaluate antibacterial activity of Dayak onion ( Eleutherine palmifolia (L) Merr) extract against fish pathogen Pseudomonas fluorescens . The method used in this study was extraction by maceration, UV-Vis spectrophotometry analysis on the extract and antibacterial activity test with Minimum Inhibitory Concentration (MIC) as well as disc diffusion test. The results of this study showed that the yield value was 8.87%. Based on UV-Vis analysis, it confirmed that extract of Dayak onion ( Eleutherine palmifolia (L) Merr) contains predominantly flavonoids and phenols with its derivatives, that have antibacterial activity. The results of MIC and disc diffusion test showed potential antibacterial activity against P. fluorescens, as observed from the value of absorbance and inhibition zones that formed. Keywords: Antibacterial, Disc diffusion, Eleutherine palmifolia (L) Merr, MIC, Pseudomonas fluorescens .
Highlights
INTRODUCTION* Pseudomonas fluorescens is a gram negative bacterium that is pathogenic to fish, causing the disease in fish [1]
Other studies have previously proven that extract of Dayak onion bulbs was able to inhibit P. fluorescens bacteria through the Minimum Inhibitory Concentration (MIC) test [7]
Dayak onion bulbs (Eleutherine palmifolia (L) Merr) obtained from UPT Materia Medica Batu were extracted by maceration and the values of the yield were calculated [9]. 100 g of simplicia were mixed with 600 mL of ethanol (PA) solvent and left to stand for three days
Summary
Dayak onion bulbs (Eleutherine palmifolia (L) Merr) obtained from UPT Materia Medica Batu were extracted by maceration and the values of the yield were calculated [9]. 100 g of simplicia were mixed with 600 mL of ethanol (PA) solvent and left to stand for three days. The concentrated extract which has been dissolved with 10% DMSO at a predetermined concentration was transferred as much as 1 mL into a test tube containing the medium at the treatment of each concentration [12]. Extract of Dayak onion with concentrations of 1000 ppm, 500 ppm, 100 ppm, 10 ppm, and 1 ppm were transferred into sterile test tubes. Each tube was inoculated with 1 mL of P. fluorescens bacterial isolate from previous liquid medium (TSB) and incubated at 37°C. Disc Diffusion Test The disc diffusion test was performed according Sekar and Rashid [13] to measure the inhibition zone occurred around the disc paper that has been given Dayak onion (Eleutherine palmifolia (L) Merr) extract. Petri dishes were incubated for 24 hours at 37°C, and the clear zones were measured using digital calipers
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