Abstract
ABSTRACT Atopic dermatitis (AD) is a common allergic, inflammatory skin disease. Here, we focused on the anti-allergic and anti-inflammatory effects of Gardenia jasminoides Ellis fructus extracts (GFE) in DNCB treated NC/Nga mice. In an in vivo study, GFE treatment significantly decreased immunoglobulin E (IgE) levels and cytokines expression in spleen and serum of treatment groups mice, compared with the negative controls (administered 0.2% DNCB only). Mice treated with GFE had fewer inflammatory cell infiltrates in the dermis and hypodermis compared to the negative controls. In addition, we also studied β-Hexosaminidase release assay and possible anti-inflammatory mechanism of GFE in RBL-2H3 cells. GFE suppressed the expression of COX-2 and TNF-α, the translocation of NF-κβ, and the phosphorylation of Syk, p38, JNK, and Erk1/2 in stimulated RBL-2H3 cells. These results strongly suggest that GFE inhibits the allergic response by reducing mast cell activation and may have therapeutic potential as an anti-atopic dermatitis agent.
Highlights
The occurrence of allergic disease has increased harmfully in the last few decades
We evaluated the cytotoxicity of Gardenia jasminoides Ellis fructus extracts (GFE) and antigen (DNP-BSA) co-treatment to find the optimal concentrations of GFE for this study
The effect of GFE on the degranulation of RBL-2H3 cells was examined. β-hexosaminidase enzyme is stored in mast cell secretory granules together with allergic mediators such as histamine. β-hexosaminidase is released while the mast cells are immunologically stimulated to degranulate, As shown in Figure 1(B), degranulation was suppressed by the addition of GFE in a dose-dependent manner
Summary
The occurrence of allergic disease has increased harmfully in the last few decades. Atopic dermatitis (AD) is an allergic inflammation disorder characterized by itchy, red, swollen, cracked skin (Hoare, Li Wan, & Williams, 2000; Leung, Boguniewicz, Howell, Nomura, & Hamid, 2004). The functions of these cells are similar to those of primary mast cells and normal basophils They are widely used to study IgE-mediated degranulation and are considered to be a good model for studying anti-allergic effects (Chung, Park, & Park, 2012; Han, Yim, An, Kim, & Kim, 1994; Marchand et al, 2003). They have been shown to promote the production of various inflammatory mediators and cytokines, including COX-2 and TNF-α (Gilfillan & Tkaczyk, 2006). These models display important features of human AD, resulting in a better understanding of the pathogenesis of this disease
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