Abstract
In this study, topical water in oil W/O emulsion containing 3% grape seed extract (GSE) was developed and evaluated its anti-aging effects in comparison with its vehicle (base) as control. Both base and selected formulation were stored at 8°C ± 0.1°C, 25°C ± 0.1°C, 40°C ± 0.1°C, 40°C ± 0.1°C with 75% relative humidity (RH) for time duration of 12 weeks in order to evaluate their physiochemical stability. With an objective to determine its anti-aging potential & safety of the formulation containing GSE, various skin parameters such as moisture level, pH value, sebum content, mean pore size, elasticity and roughness were evaluated by using non-invasive biophysical techniques. Both base and formulation were applied on human cheeks of 20 healthy female between ages of 28-58 for a period of 12 weeks. Panelists also answered a preformed questionnaire to assess performance of the product. The results of the non-invasive biophysical test have shown that the product increases skin moisture, elasticity, and smoothness significantly (p<0.05). In addition, product also decreases skin roughness and mean pore sizes significantly (p<0.05) while the base shows insignificant result (p>0.05).
Highlights
Skin provides protection against the external environment [1]
Moisturizers help normalize the protective functions of the skin and create a smooth, flexible and healthier looking skin [26] while the hydration value of the skin was 54.45 before the application of the product (T0), it was found to be 66.76, 72.92 and 80.35 after 4th, 8th and 12th weeks of application respectively by corneometer CM 825® working with capacitance principle
The results revealed that formulation increased the moisture content of skin significantly (p0.05)
Summary
Skin provides protection against the external environment [1]. It helps to maintain the fluid balance, regulates body temperature, safeguards body against the detrimental effects of sunlight, microbes and chemicals [2,3]. Intrinsic-aging is recognized as natural aging and it is chronologically emerging and inevitable process accompanied by profound genetic changes [8] It is characterized by fine wrinkles, roughness, dryness, sagging and epidermal thinning [9]. Extrinsic aging (often referred as photo-ageing) is caused due to exposure of skin to environmental factors which cause oxidative damage in skin [10]. Hyper pigmentation is characterized by dry and rough skin as well as deep wrinkles Environmental factors such as cigarette smoke, air pollution and ozone contribute to premature skin aging [11]. Ability of natural compounds having anti-oxidants when used in skin protection topical application, indicates that it may significantly slow down the process of skin-aging [15].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
