Anthocyanins, polyphenols, flavonoids and antioxidant activity in common bean (Phaseolus vulgaris L.) landraces

Elia Aquinobolaos,Yatzil Garcadaz,Jose Chavezservia,Araceli Veraguzman,Elena Herediagarcia,Jose Carrillorodrguez

doi:10.9755/ejfa.2016-02-147

Abstract

The aim of this work is to assess and describe a collection of Mexican populations of common bean (Phaseolus vulgaris L.), based on its content of monomeric anthocyanins, polyphenols, flavonoids, antioxidant activity, and grain physical characteristics. A collect of 25 bean populations was made at five states and regions in Mexico. They were cultivated in Santa Cruz Xoxocotlan, Mexico using a randomized complete blocks design with four replications. From a sample of a grain per population, polyphenols and flavonoids content were evaluated using UV-vis spectroscopy; anthocyanins content by differential pH; antioxidant activity using DPPH method, and a morphologic description of the grain was carried out. Variance analysis determined significant differences (P < 0.01) within and among population groups over all evaluated variables. Populations from Oaxaca and Puebla presented high content in anthocyanins, 1.6 and 2.1 mg C3GE/g, respectively. Polyphenols concentration was higher in seed coat (27.7 a 127.0 mg GAE/g) than in whole grain (1.3 to 5.4 mg GAE/g). Similar patterns were evaluated in flavonoids, from 5.9 to 21.5 in seed coat and 0.10 to 0.78 CE/g in grain, and in antioxidant activity (AA) from 132.5 to 1021.7 μmol ETrolox/g in seed coat. AA was significantly correlated with anthocyanins and polyphenols content. The group of outstanding populations in the evaluated compounds were: OAX-011-29, OAX-011-30, PUE-011-15, PUE-011-34, EM-01-01 and GRO-10-87. From these results we can infer that bean consumed by rural families from the central-south region of Mexico has high contents in anthocyanins, polyphenols and flavonoids with high antioxidant activity.

Highlights

Common bean (Phaseolus vulgaris L.) has a high nutritional and nutraceutical value; the grain or seed contains high contents of protein, carbohydrates, vitamins, minerals, fiber, and secondary metabolites, such as polyphenols, flavonoids, anthocyanins, carotenoids, lectins and trypsin inhibitors, among others (Reynoso-Camacho et al, 2006; GolamMasum-Akond et al, 2011; Suárez-Martínez et al, 2015)
Bean nutritional value and consumption volume have been important since pre-Columbian times for rural communities in Mexico and Latin America (Singh et al, 1991; Romero-Arenas et al, 2013; Hernández-López et al, 2013)
Mexico is the origin of common beans (Phaseolus vulgaris L.), and hundreds of local varieties and genepools of wild populations exist (Singh et al, 1991; Bitocchi et al, 2012; Hernández-López et al, 2013)

Summary

Introduction

Common bean (Phaseolus vulgaris L.) has a high nutritional and nutraceutical value; the grain or seed contains high contents of protein, carbohydrates, vitamins, minerals, fiber, and secondary metabolites, such as polyphenols, flavonoids, anthocyanins, carotenoids, lectins and trypsin inhibitors, among others (Reynoso-Camacho et al, 2006; GolamMasum-Akond et al, 2011; Suárez-Martínez et al, 2015).

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