Abstract
Tartary buckwheat (Fagopyrum tataricum )i s rich in flavonoids. Anthocyanins, a special class of flavo- noids, offer significant nutrients and provide the red pig- ment found in sprouts. The anthocyanins biosynthesis has been shown influenced by environmental stress. In this study, we investigate the effects of cold stress on antho- cyanins biosynthesis of tartary buckwheat sprouts. On cellular level, cross-sectional observations were performed and result indicated that anthocyanins accumulated pri- marily in the epidermal and cortex cells of hypocotyls. Biochemical analysis, including anthocyanin quantifica- tion, thin-layer chromatography and radical scavenging assay, showed that cold stress significantly increased the synthesis of anthocyanins and antioxidant activity of tar- tary buckwheat sprouts. At the molecular level, semi-RT- PCR was used to investigate 14 anthocyanin-related genes in tartary buckwheat sprouts treated with cold stress. All the selected genes were upregulated in cold-stressed sprouts, except for FtGST, FtAHA, and FtMYB. More importantly, the expression level of three late biosynthesis genes, FtF3 0 H, FtDFR, and FtANS, was highest in hypo- cotyl tissue. Our results suggest that anthocyanins play a considerable role in the cold stress tolerance of tartary buckwheat sprouts.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call