Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health in Mice.

Monika Martiniakova,Agnieszka Gren,Radoslav Omelka,Edyta Kapusta,Veronika Kovacova,Grzegorz Formicki,Zofia Goc,Anna Sarocka

doi:10.3390/ani10101835

Monika Martiniakova, Agnieszka Gren + Show 6 more

Open Access

https://doi.org/10.3390/ani10101835

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Abstract

Simple SummaryAlcohol consumption, the drinking of beverages containing ethanol, represents a growing problem worldwide. Alcohol intake is often combined with an improper diet based on highly processed starch products that are rich in acrylamide. Both acrylamide and alcohol have a harmful impact on bone health. We previously demonstrated that adverse effects of ethanol on cortical bone structure were partly reduced by a relatively high dose of acrylamide in mice after one remodelling cycle. The present research was designated to reveal whether the antagonistic impact of both aforementioned toxins can also be achieved using a lower dose of acrylamide. According to our results, individual administrations of acrylamide and ethanol had adverse impacts on biochemical and morphological parameters consistent with bone health in mice. However, the most detrimental effects of ethanol were again alleviated by acrylamide at the dose used in this study.The aim of present study was to verify antagonistic effect of acrylamide (AA) and ethanol (Et) on bone quality parameters. Adult mice (n = 20) were segregated into four groups following 2 weeks administration of toxins: group E1, which received AA (20 mg/kg body weight daily); group E2, which received 15% Et (1.7 g 100% Et/kg body weight daily); group E12, which received simultaneously both toxins; and a control group. An insignificant impact of individual applications of AA, Et or their simultaneous supplementation on the total body weight of mice and the length and weight of their femoral bones was identified. In group E1, higher levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), a decreased level of glutathione (GSH) and elevated endocortical bone remodelling were determined. A significantly lower relative volume of cortical bone, bone mineral density (BMD), elevated endocortical bone remodelling and cortical porosity, higher levels of ALT, AST, lower values for total proteins (TP), GSH, alkaline phosphatase (ALP), calcium, and phosphorus were recorded in group E2. In the mice from group E12, the highest endocortical bone remodelling, decreased values for BMD, TP, GSH and ALP and increased levels of ALT and AST were found. Our findings confirmed the antagonistic impact of AA and Et at doses used in this study on biochemical and morphological parameters consistent with bone health in an animal model.

Highlights

Alcohol beverages containing ethanol (Et) are widely consumed throughout the world
Mice were housed in individual flat-deck wire cages under a 12 h light/dark cycle, a temperature of 20–24 ◦ C and humidity of 55% ± 10% with free access to a standard diet (Agropol, Motycz, Poland) and water on an ad libitum basis
Enhanced levels of ALT, AST, AST, TG and GSH were in group whenE1compared compared group

Summary

Introduction

Acrylamide (AA) belongs to the most common toxins in the human diet. It is formed during frying, deep frying and baking foods rich in carbohydrates, and especially in amino acid asparagine [1]. Et on various organ systems including the skeleton have been described in several studies [2,3,4,5,6]. Their toxicity contributed to differences in selected biochemical and morphological parameters of various organs. Excessive alcohol consumption may cause several pathological conditions, such as liver failure, brain damage, and various form of cancer [8]

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