Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India

Mariathason Birdilla Selva Donio

doi:10.4172/1948-5948.1000151

Abstract

Bacillus cereus TC-1 was isolated from condenser pond of manmade solar salt pan in Thamaraikulam, Tamilnadu, India effectively suppressed the shrimp bacterial pathogens of Vibrio harveyi, V. parahaemolyticus, V. anguillarum, V. alginolyticus and V. vulnificus by In vitro antagonistic assay at 9 to 15 mm of zone of inhibition. Phylogenetic analysis and evolutionary distance revealed that, B. cereus TC-1 was highly similar to that the Bacillus sp. and other B. cereus strains. Their optimum growth rate was ranged between of 4 to 6% of NaCl in the growth media. The alkaline protease production was significantly (P< 0.001) differed among the different NaCl enriched growth media. Based on the antagonistic activity of the B. cereus TC-1 against the shrimp pathogens and its antimicrobial factors, it may be used as probiotics and developing novel antimicrobial bioactive substances against aquatic pathogens.

Highlights

Microbes from extreme environments have attracted considerable attention in recent years due to the presence of stable and novel macromolecules [1]
The present study focuses isolation, identification, biochemical characterization and antagonistic studies of the halophilic Bacillus cereus from the crystallizer pond of the solar salt works
Genomic DNA was isolated from B. cereus TC-1 strain and 100 ng was PCR amplified using 16S rRNA universal primers

Summary

Introduction

Microbes from extreme environments have attracted considerable attention in recent years due to the presence of stable and novel macromolecules [1]. They are often under extreme conditions of pressure, temperature, salinity, and depletion of micronutrients, with survival and proliferation often depending on the ability to produce biologically active compounds. Members of the Bacillus genus are often considered microbial factories for the production of a vast array of biologically active molecules potentially inhibitory for phytopathogen growth, such as kanosamine or zwittermycin A from B. cereus [6]. The present study focuses isolation, identification, biochemical characterization and antagonistic studies of the halophilic Bacillus cereus from the crystallizer pond of the solar salt works

Materials and Methods

Results and Discussion

Conclusions